Xf. Zhu et al., Study of enantioselective interactions between chiral drugs and serum albumin by capillary electrophoresis, ELECTROPHOR, 20(9), 1999, pp. 1869-1877
The separation of the enantiomers of three basic drugs, i.e., ofloxacin, pr
opranolol and verapamil, was achieved by affinity capillary electrophoresis
(ACE), with human serum albumin (HSA) and bovine serum albumin (BSA) as ch
iral selectors in phosphate buffer at pH 7.4. Ofloxacin was only separated
in the presence of BSA, and verapamil only with HSA, while propranolol was
separated with either HSA or BSA. The effects of protein concentration and
column wall adsorption on the degree of separation were investigated. Two d
isplacers, ketoprofen and warfarin, respectively, when added to the protein
containing buffer, both showed significant effects on the separation behav
ior. From these data it was argued that verapamil may bind to HSA at both l
ocations known, the warfarin binding site (I) and the ketoprofen binding si
te (II). While with BSA, binding of ofloxacin may also occur at site I, the
preferential binding site for propanolol remains controversial. A drug-dru
g interaction between propranolol and ketoprofen due to opposite charges wa
s concluded from the increase in migration time in BSA solution. The unboun
d concentration of verapamil enantiomers in solution in the presence of HSA
, as estimated from CD-modified capillary zone electrophoresis, was trigger
ed not only by the HSA concentration but also by the coadditive concentrati
on.