Micellar electrokinetic capillary chromatography of psoralen and isopsoralen

In this paper, a micellar electrokinetic capillary chromatography (MEKC) me thod to analyze the active components of psoralen and isopsoralen in the Ch inese herb Psoralea corylifolia L. was developed. It was found that the opt imum separation condition for the analysis of these compounds was a buffer solution which contained phosphate, berate and sodium dodecyl sulfate (pH 9 .2). Through optimization of the experimental condition, the two isomers co uld be baseline-separated and their contents in the methanol-extracted samp le were quantitatively analyzed.