Chronic toxicity of 2,4,6-trinitrotoluene to a marine polychaete and an estuarine amphipod

The chronic toxicity of sediment-associated 2,4,6-trinitrotoluene (TNT) to the marine polychaete Neanthes arenaceodentata and the estuarine amphipod L eptocheirus plumulosus was evaluated. Test organisms were exposed to sedime nts spiked with radiolabeled TNT for 28 d, after which time the endpoints o f mortality, growth, and reproduction (L. plumulosus only) were assayed and compared against the TNT tissue concentrations as well as the TNT sediment concentrations. Survival was significantly reduced at a tissue concentrati on of 61 mu g TNT/g wet wt tissue in N. arenaceodentata and at 6.3 mu g TNT /g wet wt tissue in L. plumulosus. The growth end point demonstrated an app arent hormesis effect in both N. arenaceodentata and L. plumulosus with enh ancement (significant for N. arenaceodentata) occurring at the lower TNT co ncentrations. Growth was significantly reduced at the highest TNT exposure of 10.0 mu g TNT/g wet wt tissue in L. plumulosus. Reproduction was signifi cantly reduced at a tissue concentration of 6.3 mu g TNT/g wet wt tissue in L. plumulosus. The results of this study demonstrate that both N. arenaceo dentata and L. plumulosus are sensitive to the presence of sediment-associa ted TNT and that more information is needed about the toxicity of TNT to be nthic fauna to facilitate risk assessment and management of TNT-contaminate d sites.