Production of a recombinant lipase artificially localized on the Bacillus subtilis cell surface

The lip B gene encoding an extracellular lipase, LipB, from Bacillus subtil is was fused to the 5'-terminal region of the cwlB gene encoding the cell w all-binding domain of the major cell wall hydrolase (autolysin). SDS-polyac rylamide gel electrophoresis and zymography revealed that the fused protein is localized in the B. subtilis cell wall fraction and retains lipase acti vity. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.