Yh. Ding et al., Four A6-TCR/peptide/HLA-A2 structures that generate very different T cell signals are nearly identical, IMMUNITY, 11(1), 1999, pp. 45-56
The interactions of three singly substituted peptide variants of the HTLV-1
Tax peptide bound to HLA-A2 with the A6 T cell receptor have been studied
using T cell assays, kinetic and thermodynamic measurements, and X-ray crys
tallography. The three peptide/MHC ligands include weak agonists and antago
nists with different affinities for TCR. The three-dimensional structures o
f the three A6-TCR/peptide/HLA-A2 complexes are remarkably similar to each
other and to the wild-type agonist complex, with minor adjustments at the i
nterface to accommodate the peptide substitutions (P6A, V7R, and Y8A). The
lack of correlation between structural changes and the type of T cell signa
ls induced provides direct evidence that different signals are not generate
d by different ligand-induced conformational changes in the alpha beta TCR.