Mutated cytochrome b as a determinant of a new monoclonal antibody (H8.98)on renal carcinoma cell lines recognized by a V gamma 3V delta 1(+) T-cellclone

We generated a monoclonal antibody (MAb), H8.98, that recognizes an antigen shared by 50% of examined renal carcinoma cell (RCC) lines and is suscepti ble to lysis by a V gamma 3V delta 1(+) T-cell clone derived from RCC tumor -infiltrating lymphocytes. H8.98 inhibited V gamma 3V delta 1(+) T-cell clo ne-mediated lysis of RCC lines. It did not stain normal kidney lines, melan omas, fibroblasts, Burkitt's lymphoma or Epstein-Barr virus-transformed B-c ell lines but it did stain 2 of 4 tested breast cancer lines, Through scree ning of a renal carcinoma cDNA library using H8.98, we isolated a cDNA clon e which, upon sequencing, was found to be cytochrome b with 2 point mutatio ns. (C) 1999 Wiley-Liss, Inc.