Routine detection of Salmonella species in water: Comparative evaluation of the ISO and PROBELIA (TM) polymerase chain reaction methods

Water is one of the main transmission routes for Salmonella spp,, the causa tive agents of salmonellosis in humans and animals. This worldwide sanitary problem requires rapid and accurate analyses to be realized so that adviso ries for exposed people are timely and reliable. Because the traditional me thod, the ISO 6340 method, does not meet the criterion of rapidity, there w as a need for a quicker method. To fulfill this need, a comparative evaluat ion between the ISO method and one based on polymerase chain reaction (PCR) , the PROBELIA, was performed, Waters from different origins were tested ei ther directly or after artificial contamination with selected Salmonella st rains isolated from the environment. The results clearly demonstrate that t he PCR-based method can advantageously replace the ISO 6340 method. It is q uicker, less labor intensive, reproducible, and provides results that match perfectly to those obtained by the ISO method. Furthermore, the PROBELIA m ethod meets the requirements for an efficient sanitary survey: high numbers of samples processed at the same time, reduced cost, and results within 2 days.