Application of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) to identification of flatfish species

Authors
Cespedes, A Garcia, T Carrera, E Gonzalez, I Fernandez, A Hernandez, PE Martin, R
Citation
A. Cespedes et al., Application of polymerase chain reaction-single strand conformational polymorphism (PCR-SSCP) to identification of flatfish species, J AOAC INT, 82(4), 1999, pp. 903-907
Citations number
56
Categorie Soggetti
Agricultural Chemistry
Journal title
JOURNAL OF AOAC INTERNATIONAL
ISSN journal
10603271 → ACNP
Volume
82
Issue
4
Year of publication
1999
Pages
903 - 907
Database
ISI
SICI code
1060-3271(199907/08)82:4<903:AOPCRS>2.0.ZU;2-N
Abstract
A method of DNA analysis based on polymerase chain reaction-single strand c onformational polymorphism (PCR-SSCP) was developed to verify the authentic ity of labeled raw and frozen fillets of some flatfish species. PCR was use d to amplify a short fragment(201 bp) of the mitochondrial cytochrome b gen e, which was denatured and analyzed by native polyacrylamide gel electropho resis for detection of SSCPs, Species-specific patterns of DNA bands were o btained for sole (Solea solea), European plaice (Pleuronectes platessa), fl ounder (Platichthys flesus) and Greenland halibut (Reinhardtius hippoglosso ides).