Application of microbial receptor assay to quantitation of residues of spectinomycin in bovine milk and comparison with liquid chromatographic analysis

Spectinomycin-contaminated bovine milk samples were assayed by liquid chrom atographic (LC) and microbial receptor methods. LC involved a newly develop ed analytical method to quantitate the concentration of spectinomycin in th e contaminated milk samples. The receptor assay used reagents and the react ion system used for the Charm II spectinomycin assay. Three standard curves (selected range, full range, and second-order polynomial) were plotted for the receptor assay and used to quantitate spectinomycin in contaminated mi lk samples. The levels of spectinomycin obtained by the receptor assay, usi ng only the standard curve in the selected range, were comparable to the re sults obtained by LC analysis.