Application of microbial receptor assay to quantitation of residues of spectinomycin in bovine milk and comparison with liquid chromatographic analysis
B. Shaikh et al., Application of microbial receptor assay to quantitation of residues of spectinomycin in bovine milk and comparison with liquid chromatographic analysis, J AOAC INT, 82(4), 1999, pp. 1002-1005
Spectinomycin-contaminated bovine milk samples were assayed by liquid chrom
atographic (LC) and microbial receptor methods. LC involved a newly develop
ed analytical method to quantitate the concentration of spectinomycin in th
e contaminated milk samples. The receptor assay used reagents and the react
ion system used for the Charm II spectinomycin assay. Three standard curves
(selected range, full range, and second-order polynomial) were plotted for
the receptor assay and used to quantitate spectinomycin in contaminated mi
lk samples. The levels of spectinomycin obtained by the receptor assay, usi
ng only the standard curve in the selected range, were comparable to the re
sults obtained by LC analysis.