Toxicity of fatty acid 18 : 5n3 from Gymnodinium cf. mikimotoi: II. Intracellular pH and KC uptake in isolated trout hepatocytes

Authors
Fossat, B Porthe-Nibelle, J Sola, P Masoni, A Gentien, P Bodennec, G
Citation
B. Fossat et al., Toxicity of fatty acid 18 : 5n3 from Gymnodinium cf. mikimotoi: II. Intracellular pH and KC uptake in isolated trout hepatocytes, J APPL TOX, 19(4), 1999, pp. 275-278
Citations number
16
Categorie Soggetti
Pharmacology & Toxicology
Journal title
JOURNAL OF APPLIED TOXICOLOGY
ISSN journal
0260437X → ACNP
Volume
19
Issue
4
Year of publication
1999
Pages
275 - 278
Database
ISI
SICI code
0260-437X(199907/08)19:4<275:TOFA1:>2.0.ZU;2-W
Abstract
Effects of octadecapentaenoic acid 18:5n3 and other related polyunsaturated fatty acids present in Gymnodinium cf. mikimotoi were tested in isolated t rout hepatocytes, These exotoxins decreased intracellular pH followed by a slow recovery to initial value and alkalinization of acidic compartments, s uggesting an inhibition of vacuolar H+-ATPases. Moreover, addition of 18:5n 3 to the extracellular medium induced a decrease of K+ uptake into hepatocy tes as a result of Na,K-ATPase inhibition. However, high concentrations (10 (-5)-10(-3) M) are necessary to induce these effects. Copyright (C) 1999 Jo hn Wiley & Sons, Ltd.