Cloning, sequence, and transcriptional regulation of the operon encoding aputative N-acetylmannosamine-6-phosphate epimerase (nanE) and sialic acid lyase (nanA) in Clostridium perfringens

Clostridium perfringens can obtain sialic acid from host tissues by the act ivity of sialidase enzymes on sialoglycoconjugates. After sialic acid is tr ansported into the cell, sialic acid lyase (NanA) then catalyzes the hydrol ysis of sialic acid into pyruvate and N-acetylmannosamine. The latter is co nverted for use as a biosynthetic intermediate or carbohydrate source in a pathway including an epimerase (NanE) that converts N-acetylmannosamine-6-p hosphate to N-acetylglucosamine-6-phosphate. A 4.0-kb DNA fragment from C. perfringens NCTC 8798 that contains the nanE and nanA genes has been cloned . The identification of the nanA gene product as sialic acid lyase was conf irmed by overexpressing the gene and measuring sialic acid lyase activity i n a nanA Escherichia coli strain, EV78. The nanA gene product was also show n to restore growth to EV78 in minimal medium with sialic acid as the sole carbon source. By using Northern blot experiments, it was demonstrated that the nanE and nanA genes comprise an operon and that transcription of the o peron in C. perfringens is inducible by the addition of sialic acid to the growth medium. The Northern blot experiments also showed that there is no c atabolite repression of nanE-nanA transcription by glucose. With a plasmid construct containing a promoterless cpe-gusA gene fusion, in which beta-glu curonidase activity indicated that the gusA gene acted as a reporter for tr anscription, a promoter was localized to the region upstream of the nanE ge ne. Primer extension experiments then allowed us to identify a sialic acid- inducible promoter located 30 bp upstream of the nanE coding sequence.