Simultaneous determination of Psi and Phi angles in proteins from measurements of cross-correlated relaxation effects

Authors
Pelupessy, P Chiarparin, E Ghose, R Bodenhausen, G
Citation
P. Pelupessy et al., Simultaneous determination of Psi and Phi angles in proteins from measurements of cross-correlated relaxation effects, J BIOM NMR, 14(3), 1999, pp. 277-280
Citations number
13
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF BIOMOLECULAR NMR
ISSN journal
09252738 → ACNP
Volume
14
Issue
3
Year of publication
1999
Pages
277 - 280
Database
ISI
SICI code
0925-2738(199907)14:3<277:SDOPAP>2.0.ZU;2-M
Abstract
A method is presented to determine both phi and psi backbone angles in prot eins simultaneously. This is achieved by measuring the effect on two-spin c oherences of cross-correlation between N-15-H-1(N) and C-13(alpha)-H-1(alph a) vectors. The cross-correlation rates are obtained by comparing two compl ementary three-dimensional experiments.