Isolation of measles virus from a naturally-immune, asymptomatically re-infected individual

Background:The changing epidemiology of measles with mild measles cases inc reasingly being recognised in previously-vaccinated individuals, suggests t hat more asymptomatic or subclinical cases might be occurring. Although thi s has been clearly documented in previously-vaccinated individuals, the fre quency of these asymptomatic infections in individuals previously naturally -infected with measles is not known. Also, it is not known whether these as ymptomatic or mildly-infected individuals who do not display the full range of clinical signs of measles are capable of transmitting the virus to othe r susceptible persons. Objectives: To demonstrate the isolation of measles virus (MV) from previou sly, naturally-immune individuals asymptomatically infected with measles wh ile in close contact with acutely infected family members and to document t he secondary immune responses (SIR) associated with asymptomatic measles in fection. Study Design: Throat swab and urine specimens from five acute measles cases and their family contacts, taken within 5 days of onset of rash in each ac ute case, were used to isolate MV by tissue culture. Positive tissue cultur e results were confirmed by indirect immunofluorescence (IF) staining. Meas les specific antibodies (IgG and IgM), IgG urea avidity and measles-neutral ising antibodies were measured in the one family (index family)where an asy mptomatic measles infection of a contact was demonstrated. Results: The acutely infected patient in the index family (T1/96) had a mea sles-neutralising antibody titre of < 1:10, measles IgG urea avidity of 24% and MV was isolated and confirmed by IF from urine and throat swab specime ns. T1/96 represents acute measles infection after primary vaccine failure because he had a clear history of being vaccinated against measles as a chi ld. MV was also successfully isolated from throat swab and urine specimens from the other four acute cases and from the urine but not the throat swab of an asymptomatically infected family contact in the index family (mother, T2/96). T2/96 had a history of natural measles infection as a child approx imately 50 years ago. In addition to detectable MV in urine this contact al so had a SIR with a rise in measles specific neutralising antibody titre. N o virus was isolated from the other contact in the index family (father, T3 /96) or from the contacts of the other four acute cases examined. Conclusions: This is the first report of a confirmed asymptomatic MV infect ion, by MV isolation and IF testing and a concurrent SIR, in a previously n aturally-immune contact of an acute case. The importance of these findings to the epidemiology and control of MV as well as the diagnostic value of MV urine isolation and IF confirmation for mild or asymptomatic cases must be examined further. (C) 1999 Elsevier Science B.V. All rights reserved.