Magnetic capture-hybridization method for purification and probing of mRNAfor neutral protease of Bacillus cereus

A magnetic capture-hybridization method was assessed for the isolation of p rokaryotic mRNA for the neutral protease of B. cereus from liquid culture. A biotin-labeled specific probe was hybridized to the mRNA transcripts and subsequently captured by streptavidin-coated paramagnetic beads. mRNA was d etected by dot-blot hybridization with a ds DIG-labeled DNA-probe. The magn etic capture hybridization is a rapid and simple method and has a promising potential for gene expression studies in complex samples. (C) 1999 Elsevie r Science B.V. All rights reserved.