CLONING, CHARACTERIZATION, AND SEQUENCE-ANALYSIS OF THE CLCE GENE ENCODING THE MALEYLACETATE REDUCTASE OF PSEUDOMONAS SP STRAIN B13

Authors
KASBERG T SEIBERT V SCHLOMANN M REINEKE W
Citation
T. Kasberg et al., CLONING, CHARACTERIZATION, AND SEQUENCE-ANALYSIS OF THE CLCE GENE ENCODING THE MALEYLACETATE REDUCTASE OF PSEUDOMONAS SP STRAIN B13, Journal of bacteriology, 179(11), 1997, pp. 3801-3803
Citations number
20
Categorie Soggetti
Microbiology
Journal title
Journal of bacteriology → ACNP
ISSN journal
00219193
Volume
179
Issue
11
Year of publication
1997
Pages
3801 - 3803
Database
ISI
SICI code
0021-9193(1997)179:11<3801:CCASOT>2.0.ZU;2-9
Abstract
A 3,167-bp PstI fragment of genomic DNA from Pseudomonas sp. strain B1 3 nas cloned and sequenced. The gene clcE consists of 1,059 nucleotide s encoding a protein of 352 amino acids with a calculated mass of 37,7 69 Da which showed maleylacetate reductase activity. The protein had s ignificant sequence similarities with the polypeptides encoded by tcbF of pP51 (59.4% identical positions), tfdF of pJP4 (55.1%), and tftE o f Burkholderia cepacia AC1100 (53.1%). The function of TcbF as maleyla cetate reductase was established by an enzyme assay.