A first-generation porcine whole-genome radiation hybrid map

A whole-genome radiation hybrid (WG-RH) panel was used to generate a first- generation radiation map of the porcine (Sus scrofa) genome. Over 900 Type I and II markers were used to amplify the INRA-University of Minnesota porc ine Radiation Hybrid panel (IMpRH) comprised of 118 hybrid clones. Average marker retention frequency of 29.3% was calculated with 757 scorable marker s. The RHMAP program established 128 linkage groups covering each chromosom e (n = 19) at a lod greater than or equal to 4.8. Fewer than 10% of the mar kers (59) could not be placed within any linkage group at a lod score great er than or equal to 4.8. Linkage group order for each chromosome was determ ined by incorporating linkage data from the swine genetic map as well as ph ysical assignments. The current map has an estimated ratio of similar to 70 kb/cR and a maximum theoretical resolution of 145 kb. This initial map for ms a template for establishing accurate YAC and BAC contigs and eventual po sitional cloning of genes associated with complex traits.