Biological weighting of ultraviolet (280-400 nm) induced mortality in marine zooplankton and fish. II. Calanus finmarchicus (Copepoda) eggs

The copepod Calanus finmarchicus Gunnerus is a key component of the plankto nic food web in the Gulf of St. Lawrence, Canada. In this region, productiv ity-determining biophysical interactions occur in the upper 0 to 30 m of th e water column. The eggs and nauplii of C. finmarchicus are found in this l ayer. Measurements of the diffuse attenuation coefficients for solar ultrav iolet-B radiation (280 to 320 nm, UV-B) at various locations in this region indicated maximum 10% depths (the depth to which 10% of the surface energy penetrates) of 3 to 4 m at a wavelength of 310 nm. This represents a signi ficant percentage of the summer mixed-layer water column: organisms residin g in this layer are exposed to UV-B radiation. Laboratory experiments using a Xenon-arc-lamp based solar simulator revealed that C. finmarchicus embry os exposed to UV-B exhibited high wavelength-dependent mortality. The stron gest effects occurred under exposures to wavelengths below 312 nm. A signif icant percentage of nauplii hatched from eggs exposed to these wavelengths exhibited malformations indicative of errors in pattern formation during em bryogenesis. At the shorter wavelengths (<305 nm), UV-B-induced mortality w as strongly dependent on cumulative exposure. The biological weighting func tion (BWF) derived for UV-B-induced mortality in C. finmarchicus eggs is si milar to that reported for naked DNA. This suggests that the UV-B-induced m ortality effect on C. finmarchicus embryos is a direct result of DNA damage . There was no evidence of a detrimental effect of ultraviolet-A radiation (320 to 400 nm). Calculations based upon the BWF indicate that, under curre nt noon surface irradiance, 50% of C. finmarchicus eggs located at or very near (within 10 cm) the ocean surface will be dead after 2.5 h of exposure. Under solar spectral irradiance simulating a 20% decrease in ozone layer t hickness, this time drops to 2.2 h. These are first-order estimates based u pon irradiance taken at a time of day during which the values would be maxi mal. Nonetheless, they illustrate the relative changes in UV-B effects that will result from ozone layer depletions expected over the coming decades. It is also important to point out that variability in cloud cover, water qu ality, and vertical distribution and displacement within the mixed layer, c an all have a greater effect on the flux of UV-B radiation to which C. finm archicus eggs are exposed than will ozone layer depletion at these latitude s.