The synergistic interactions of oleoyl-lysophosphatidic acid in platelet aggregation

We have studied the ability of oleoyl-lysophosphatidic acid (O-LPA) to indu ce a synergistic platelet aggregatory response with the following agents: A DP, noradrenaline (NA), thrombin receptor agonist peptide (TRAP1-6), arachi donic acid (AA), phorbol 12-myristate 13-acetate (PMA), Ca2+ ionophore A23, 187 and sodium fluoride (NaF). O-LPA mediated synergism with the aggregatin g agents (ADP, noradrenaline and TRAP1-6) which mediate their aggregatory r esponses through platelet membrane-bound receptors. Arachidonic acid also f alls into the above group, as the platelet aggregation mediated by this fat ty acid is through the synthesis of thromboxane A, which binds to its own p latelet cell membrane receptor. O-LPA reduced the response time of platelet s to both PMA and the Ca2+ ionophore A23,187 with minimum effect on the tot al percentage aggregation. Finally, O-LPA appeared not to synergise with Na F. Med Sci Res 27:435-441 (C) 1999 Lippincott Williams & Wilkins.