Molecular phylogeny of Amanita based on large-subunit ribosomal DNA sequences: implications for taxonomy and character evolution

Phylogenetic relationships in the genus Amanita were investigated using seq uence data fr-om the nuclear-encoded large subunit ribosomal DNA. Exemplar taxa were selected to represent the all sections from the current classific ations of Singer, Bas and Jenkins. Phylogenetic analysis of the nuc-LSU reg ion supports the taxonomic distinction of subgenera Amanita and Lepidella, as well as all nine sections previously recognized by Singer (R. Singer, 19 86, The Agaricales in Modern Taxonomy, 4th ed.) including sections Amanita, Amidellae, Caesareae, Lepidella, Mappae, Phalloideae, Ovigerae, Vaginatae, Validae. Phylogenetic analyses among these nine terminal groups reveal sev eral higher-level relationships which are also supported by morphological a nd biochemical characters. A phylogenetic classification is proposed which recognizes two subgenera (Amanita, Lepidella), four sections (Amanita, Vagi nate, Phalloideae, Lepidella), seven subsections (Amanita, Ovigerae, Amidel lae, Caesareae, Phalloideae, Vaginatae, Validae), and two series (Mappae, V alidae).