Jw. Mozrzymas et al., Facilitation of miniature GABAergic currents by chlorpromazine in culturedrat hippocampal cells, NEUROREPORT, 10(11), 1999, pp. 2251-2254
THE whole cell configuration of the patch clamp technique was used to study
the effects of chlorpromazine (CPZ), a widely used antipsychotic drug, on
miniature GABAA-mediated synaptic currents (mIPSCs) in hippocampal cells in
culture. CPZ (10-30 mu M) induced a clear dose-dependent increase of mIPSC
s frequency that was associated with a decrease in amplitude and with an ac
celeration of their decay kinetics. When applied in a calcium-free medium,
CPZ was less effective in enhancing mIPSCs frequency, suggesting that this
effect was partially calcium dependent. While a low (10 mu M) CPZ concentra
tion induced a 2-fold increase in the total charge transfer a higher (30 mu
M) dose of this drug produced no changes, indicating that the presynaptic
effect was counterbalanced by the postsynaptic one. NeuroReport 10:2251-225
4 (C) 1999 Lippincott Williams & Wilkins.