Cellular calcium handling in brain slices from calbindin D-28k-deficient mice

CELLULAR calcium handling was examined in brain slices from transgenic anti sense mice with a regional deficiency in the neuronal calcium binding prote in calbindin D-28k and from their non transgenic wild type litter mate cont rols. Depolarization of brain slices with NMDA or potassium produced a prol onged elevation of neuronal calcium signal in neurons in brain slices from calbindin D-28k-deficient transgenic mice. This effect was selective and wa s seen only in brain areas where the antisense construct produced a signifi cant depletion of calbindin D-28k protein. In other regions where calbindin D28k protein was not modified by the construct and in all glial cells whet her from wild type or transgenic mice, cellular calcium handling was normal . NeuroReport 10:2367-2372 (C) 1999 Lippincott Williams & Wilkins.