Growth inhibitory effects of somatostatin on human leukemia cell lines mediated by somatostatin receptor subtype 1

Reverse transcription polymerase chain reaction analysis revealed that only somatostatin receptor (SSTR) 1 mRNA was expressed in Ball-1 B-, Jurkat T-, and HL60 leukemia cell lines. In contrast, human normal mononuclear cells expressed the mRNA of all five subtypes of SSTR, although the expression le vel of SSTR1 was the highest. A binding study, revealed that [I-125]-somato statin bound specifically to HL60 cells and this binding was inhibited conc entration-dependently by unlabeled somatostatin (SS). A [H-3]thymidine inco rporation study showed that SS significantly and concentration-dependently inhibited HL60 and BALL-1 leukemia cell growth. Furthermore, this inhibitio n of leukemia cell growth was associated with reduces c-fos gene expression . These data indicate that leukemia cells express SSTR1 and SS reduce c-fos gene expression with resultant suppression of leukemia cell growth, possib ly mediated by the SSTR1. (C) 1999 Elsevier Science Inc. All rights reserve d.