The effects of cisplatinum and vincristine on peripheral nerve regeneration

Current treatment modalities for extremity sarcoma often include tumor exti rpation plus neoadjuvant therapy. Limb-sparing surgery may require reconstr uction of critical nerve defects. Neurotoxic side effects from adjuvant che motherapy have been reported and raise concerns regarding the effects of ch emotherapy on nerve regeneration. In an attempt to define the effects of ad juvant chemotherapy on peripheral nerve regeneration, cisplatin and vincris tine were administered to rats following isografting of the posterior tibia l nerve. Parameters used to assess peripheral nerve regeneration included w alking track analysis and histomorphology. Sixty 250-g Sprague-Dawley rats were randomly allocated into one of three t reatment groups. Each animal underwent a 15-mm reversed interposition nerve isograft from 30 donor rats into the right posterior tibial nerve. Ten ani mals sen ed as control. The remaining animals were divided into two groups of 25 animals each. One group received cisplatin (75 mg/m(2)) and the other group received vincristine (1 mg/m(2)). Chemotherapy was administered at 4 -week cycles for a total of six cycles (24 weeks). Walking track analysis w as performed monthly. Nerve specimens were harvested from the grafted segme nt and the distal posterior tibial nerve for histomorphology. Walking track analysis demonstrated no statistical difference in print leng th between the control and chemotherapeutic groups at the conclusion of the study. The number of axons per square millimeter and nerve fiber density w ere not statistically different between control and chemotherapeutic groups . In the rodent posterior tibial nerve model, postoperative adjuvant therapy does not significantly alter functional outcome in peripheral nerve regener ation. The practice of immediate nerve grafting after tumor extirpation, de spite planned postoperative chemotherapy, is sup ported.