Disruption of six Saccharomyces cerevisiae novel genes and phenotypic analysis of the deletants

As a part of the EUROFAN programme, six open reading frames from Saccharomy ces cerevisiae (YNL083w, YNL086w, YNL087w, YNL097c, YDL100c and YOR086c) we re disrupted in two genetic backgrounds, FY1679 and W303. Individual deleti ons in diploid strains and tetrad analysis of heterozygous deletants reveal ed that none of them is essential. Basic phenotypic analysis did not reveal any significant difference between the parental and mutant strains. Althou gh YNL087w and YOR086c are 55% identical, the double disruptant also behave s the same as the parental cells. Ydl100p seems to be involved in metal det oxification, the phenotype of the null mutants being enhanced when the assa ys are performed at 37 degrees C. Copyright (C) 1999 John Wiley & Sons, Ltd .