C. Martin et al., Pregnant rat myometrial cells show heterogeneous ryanodine- and caffeine-sensitive calcium stores, AM J P-CELL, 46(2), 1999, pp. C243-C252
Intracellular Ca2+ release channels such as ryanodine receptors play crucia
l roles in the Ca2+-mediated signaling that triggers excitation-contraction
coupling in muscles. Although the existence and the role of these channels
are well characterized in skeletal and cardiac muscles, their existence in
smooth muscles, and more particularly in the myometrium, is very controver
sial. We have now clearly demonstrated the expression of ryanodine receptor
Ca2+ release channels in rat myometrial smooth muscle, and for the first t
ime, intracellular Ca2+ concentration experiments with indo 1 on single myo
metrial cells have revealed the existence of a functional ryanodine- and ca
ffeine-sensitive Ca2+ release mechanism in 30% of rat myometrial cells. RT-
PCR and RNase protection assay on whole myometrial smooth muscle demonstrat
e the existence of all three ryr mRNAs in the myometrium: ryr3 mRNA is the
predominant subtype, with much lower levels of expression for ryr1 and ryr2
mRNAs, suggesting that the ryanodine Ca2+ release mechanism in rat myometr
ium is largely encoded by ryr3. Moreover, using intracellular Ca2+ concentr
ation measurements and RNase protection assays, we have demonstrated that t
he expression, the percentage of cells responding to ryanodine, and the fun
ction of these channels are not modified during pregnancy.