Identification of functional amino acids in the macrolide 2 '-phosphotransferase II

Authors
Taniguchi, K Nakamura, A Tsurubuchi, K Ishii, A O'Hara, K Sawai, T
Citation
K. Taniguchi et al., Identification of functional amino acids in the macrolide 2 '-phosphotransferase II, ANTIM AG CH, 43(8), 1999, pp. 2063-2065
Citations number
24
Categorie Soggetti
Microbiology
Journal title
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
ISSN journal
00664804 → ACNP
Volume
43
Issue
8
Year of publication
1999
Pages
2063 - 2065
Database
ISI
SICI code
0066-4804(199908)43:8<2063:IOFAAI>2.0.ZU;2-D
Abstract
Macrolide 2'-phosphotransferase [MPH(2')] transfers the gamma phosphate of ATP to the 2'-OH group of macrolide antibiotics. The role of aspartic acids in the putative ATP-binding site of MPH(2')II was investigated through the substitution of alanine for aspartate by site-directed mutagenesis. D200A, D209A, D219A, and D231A mutant strains were unable to inactivate the subst rate oleandomycin, while a D227A mutant retained 7% of the activity of the original enzyme.