Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR

Authors
Romero, C Lopez-Goni, I
Citation
C. Romero et I. Lopez-goni, Improved method for purification of bacterial DNA from bovine milk for detection of Brucella spp. by PCR, APPL ENVIR, 65(8), 1999, pp. 3735-3737
Citations number
13
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
8
Year of publication
1999
Pages
3735 - 3737
Database
ISI
SICI code
0099-2240(199908)65:8<3735:IMFPOB>2.0.ZU;2-N
Abstract
Different methods of extraction of bacterial DNA from bovine milk to improv e the direct detection of Brucella by PCR were evaluated. We found that the use of a lysis buffer with high concentrations of Tris, EDTA, and NaCl, hi gh concentrations of sodium dodecyl sulfate and proteinase K, and high temp eratures of incubation was necessary for the efficient extraction of Brucel la DNA. The limit of detection by PCR was 5 to 50 Brucella CFU/ml of milk.