Quantification of the flavonoid-degrading bacterium Eubacterium ramulus inhuman fecal samples with a species-specific oligonucleotide hybridization probe

Authors
Simmering, R Kleessen, B Blaut, M
Citation
R. Simmering et al., Quantification of the flavonoid-degrading bacterium Eubacterium ramulus inhuman fecal samples with a species-specific oligonucleotide hybridization probe, APPL ENVIR, 65(8), 1999, pp. 3705-3709
Citations number
35
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
8
Year of publication
1999
Pages
3705 - 3709
Database
ISI
SICI code
0099-2240(199908)65:8<3705:QOTFBE>2.0.ZU;2-1
Abstract
To investigate the occurrence of the flavonoid-degrading bacterium Eubacter ium ramulus in the human intestinal tract, an oligonucleotide probe designa ted S-S-E.ram-0997-a-A-18 was designed and validated, with over 90 bacteria l strains representing the dominant described human fecal flora. Applicatio n of S-S-E.ram-0997-a-A-18 to fecal samples from 20 subjects indicated the presence off. ramulus in each individual tested in numbers from 4.4 x 10(7) to 2.0 x 10(9) cells/g of fecal dry mass. Six fecal E. ramulus isolates we re recognized by S-S-E.ram-0997-a-A-18 but exhibited different band pattern s when analyzed by randomly amplified polymorphic DNA.