High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis

Authors
Hausner, M Wuertz, S
Citation
M. Hausner et S. Wuertz, High rates of conjugation in bacterial biofilms as determined by quantitative in situ analysis, APPL ENVIR, 65(8), 1999, pp. 3710-3713
Citations number
32
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
8
Year of publication
1999
Pages
3710 - 3713
Database
ISI
SICI code
0099-2240(199908)65:8<3710:HROCIB>2.0.ZU;2-J
Abstract
Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy follo wed by three-dimensional analysis of cellular biovolumes revealed conjugati on rates 1,000-fold higher than those determined by classical plating techn iques. Conjugation events were not affected by nutrient concentration alone but were influenced by time and biofilm structure.