The spheroplast lysis assay for yeast in microtiter plate format

Authors
Ovalle, R Spencer, M Thiwanont, M Lipke, PN
Citation
R. Ovalle et al., The spheroplast lysis assay for yeast in microtiter plate format, APPL ENVIR, 65(8), 1999, pp. 3325-3327
Citations number
29
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
00992240 → ACNP
Volume
65
Issue
8
Year of publication
1999
Pages
3325 - 3327
Database
ISI
SICI code
0099-2240(199908)65:8<3325:TSLAFY>2.0.ZU;2-H
Abstract
A yeast lysis assay in the microtiter plate format improved precision and t hroughput and led to an improved algorithm for estimating lag time. The ass ay reproducibly revealed differences of 10% or greater in the maximal lysis rate and 50% or greater in the lag time. Clonal differences were determine d to be the major source of variation. Microtiter-based assays should be us eful for screening for drug susceptibility and for analyzing mutant phenoty pes.