A. Mariscal et al., Development and testing of a microbiological assay to detect residual effects of disinfectant on hard surfaces, APPL ENVIR, 65(8), 1999, pp. 3717-3720
We describe a glucuronidase bioassay for detecting residual bactericidal ac
tivity from the use of disinfectants on hard surfaces; in this assay we use
d formaldehyde, ethanol, isopropanol, chlorine, and a commercial preparatio
n containing 2-bromo-2-nitro-1,3-propanediol. Chlorine and the commercial p
reparation showed bactericidal activity (53.5% and 98.2%, respectively) for
a week after disinfection.