A comparison of iron-sulfur proteins obtained from Thiobacillus ferrooxidan
s was carried out. The microorganisms were grown on iron(II)- or sulfur-con
taining nutrients. In both cases different, broad elctron paramagnetic reso
nance (EPR) lines, originating from an iron(III) compound, were detected. A
dditional EPR lines of tetrahedral iron(III) and free radicals were observe
d. The UV spectra of these compounds also differ.