Relationships between antioxidants, antioxidant enzyme activities and lipid peroxidation products during early development in Dentex dentex eggs and larvae

The overall aim is the characterization of the antioxidant systems in marin e fish during early development in order to enhance growth and quality of e arly life stages by avoiding oxidation problems that may cause pathologies and disease. The present study determined the activities of the antioxidant system enzymes and levels of vitamin E, lipid peroxidation products and fa tty acids during early development without exogenous feeding in the marine fish, Dentex dentex. Samples of fertilized eggs just prior to hatch, newly hatched larvae and unfed larvae up to day 9 after hatch were analysed on a daily basis. The larvae did not grow during the experiment and by day 9 the surviving larvae weighed only a third of the newly hatched larvae. Lipid w as a major energy source during this period of starvation as evidenced by g reatly decreased amounts of total lipid due to a preferential utilisation o f neutral lipids. All groups of fatty acids (saturated, monounsaturated and polyunsaturated) were consumed throughout the starvation period. Vitamin E levels were relatively constant until significantly declining by days 8 an d 9. Similarly, levels of lipid-soluble fluorescent products (LSFP) were re latively constant until day 6 and then significantly decreased from days 7 to 9. In contrast, the level of malondialdehyde (MDA) was very high in eggs but was 3-fold lower in newly hatched larvae and then decreased over the w hole time-course and was also significantly lower on days 8 and 9. The acti vities of enzymes of the antioxidation system were expressed in both eggs a nd early larvae and varied during the developmental period with levels of c atalase and glutathione peroxidase (GPX) being initially low in eggs wherea s glutathione-S-transferase (GST) and superoxide dismutase (SOD) were highe r in eggs than in larvae. Overall, the activities of catalase and GPX incre ased, whereas GST and total SOD activities decreased, during the early stag es of development during a period where there was no exogenous dietary inpu t into the larvae. Glutathione reductase activity was very low and showed a n increasing trend initially until days 2-3 before declining and varying ov er the remaining period. In conclusion, we determined the prooxidant (polyu nsaturated fatty acid) status, antioxidant status (vitamin E), peroxidation status (MDA and LSFP) and the activities of the antioxidant enzyme systems in a single integrated study in unfed marine fish larvae during early deve lopment. (C) 1999 Elsevier Science B.V. All rights reserved.