Cy. Chu et al., Protective effects of capillarisin on tert-butylhydroperoxide-induced oxidative damage in rat primary hepatocytes, ARCH TOXIC, 73(4-5), 1999, pp. 263-268
Capillarisin (Cap), a main constituent of Artemisia capillaris (Compositae)
, was studied for its antioxidant bioactivity. In the preliminary study, Ca
p expressed a antioxidant property by its capacity for quenching the free r
adicals of 1,1-diphenyl-2-picrylhydrazyl (DPPH). This antioxidant bioactivi
ty of Cap was investigated further using a model of t-butyl-hydroperoxide (
t-BHP)-induced cytotoxicity and genotoxicity in rat primary hepatocytes. Re
sults presented here demonstrate that Cap, at concentrations of 0.01-1.00 m
g/ml, significantly decreased the leakage of lactate dehydrogenase (LDH) an
d alanine aminotransferase (ALT) and the formation of malondialdehyde (MDA)
induced by 30 min treatment of t-BHP (1.5 mM) in primary cultured rat hepa
tocytes. Cap also attenuated the t-BHP-induced diminution of glutathione (G
SH) and high level of DNA repaired synthesis. These results lead to specula
tion that Cap presents inhibitory effects against t-BHP-caused cytotoxicity
and genotoxicity in rat primary hepatocyte cultures at least via two disti
nct pathways, stabilizing the GSH system and quenching free radicals.