Sequence analysis of RNA-2 of different isolates of broad bean wilt virus confirms the existence of two distinct species - Brief report

Citation
Yo. Kobayashi et al., Sequence analysis of RNA-2 of different isolates of broad bean wilt virus confirms the existence of two distinct species - Brief report, ARCH VIROL, 144(7), 1999, pp. 1429-1438
Citations number
12
Categorie Soggetti
Microbiology
Journal title
ARCHIVES OF VIROLOGY
ISSN journal
03048608 → ACNP
Volume
144
Issue
7
Year of publication
1999
Pages
1429 - 1438
Database
ISI
SICI code
0304-8608(1999)144:7<1429:SAOROD>2.0.ZU;2-K
Abstract
The complete nucleotide sequence of RNA-2 from a Japanese isolate IP of bro ad bean wilt virus (BBWV) was determined. The sequence encodes a single lar ge polyprotein, which contains a putative movement protein and two coat pro teins (CPs). The 3'-terminal sequences of RNA-2 were also determined for th ree other Japanese isolates and two ATCC isolates (PV132 and PV176) of BBWV . The CPs of the four Japanese isolates share 86.8-98.0% amino acid sequenc es homology with one another and 88.3-96.5% with those reported for the iso late PV131 (BBWV-2). However, they have only 57.9-66.2% homology with those of PV132 and PV176 (BBWV-1).