Sequence analysis of RNA-2 of different isolates of broad bean wilt virus confirms the existence of two distinct species - Brief report

The complete nucleotide sequence of RNA-2 from a Japanese isolate IP of bro ad bean wilt virus (BBWV) was determined. The sequence encodes a single lar ge polyprotein, which contains a putative movement protein and two coat pro teins (CPs). The 3'-terminal sequences of RNA-2 were also determined for th ree other Japanese isolates and two ATCC isolates (PV132 and PV176) of BBWV . The CPs of the four Japanese isolates share 86.8-98.0% amino acid sequenc es homology with one another and 88.3-96.5% with those reported for the iso late PV131 (BBWV-2). However, they have only 57.9-66.2% homology with those of PV132 and PV176 (BBWV-1).