Quantification of cellular properties from external fields and resulting induced velocity: Cellular hydrodynamic diameter

An experimental technique is discussed in which the size distribution of a population of cells is determined by calculating each cell's settling veloc ity. The settling velocity is determined from microscopically obtained imag es which were recorded on SVHS tape. These images are then computer imaged and processed, and the cell's location and velocity are determined using a computer algorithm referred to as cell tracking velocimetry (CN). Experimen tal data is presented comparing the distribution of human lymphocytes and a human breast cancer cell line, MCF-7, determined using a Coulter counter a nd the CTV approach. (C) 1999 John Wiley & Sons, Inc.