Identification of a hexasaccharide sequence able to inhibit thrombin and suitable for 'polymerisation'

Three hexasaccharides, having from low to very high affinity for antithromb in, were synthesised from disaccharide building block precursors. One of th em, methyl(sodium 2,3-di-O-methyl-4-O-sodium sulfonato-alpha-L-idopyranosyl uronate)-(1 --> 4)-[(2,3,6-tri-O-sodium sulfonato-alpha-D-glucopyranosyl)-( 1 --> 4)-(sodium 2,3-di-O-methyl-alpha-L-idopyranosyluronate)-(1 --> 4)](2) -2,3,6-tri-O-sodium sulfonato-alpha-D-glucopyranoside, obtainable from a si ngle disaccharide building block precursor, constitutes a good starting poi nt for obtaining simple oligosaccharidic heparin mimetics able to inhibit t he two coagulation factors thrombin and Factor Xa. (C) 1999 Elsevier Scienc e Ltd. All rights reserved.