Studies on the fluorescence enhancement of reverse micelle on 2,3-diaminophenazine

The effects of reverse micelle (AOT/n-octane) on spectral characteristics o f 2,3-diaminophenazine (DAP), which was an oxidized product of o-phenylened iamine catalyzed by laccase, were investigated. It was shown that there was little effect on the absorption features of the product by AOT/n-octane. H owever the fluorescence of the product was greatly enhanced by AOT/n-octane , and the quantum yield was increased about 30-folds by AOT/n-octane. The e ffects of microenvironment on the fluorescent property of DAP and the mecha nism of fluorescence enhancement in reverse micelle AOT/12-octane were also studied.