Resource partitioning has been intensely studied in fish assemblages o
f tropical reefs, whereas surprisingly little research has been conduc
ted on the benthic Mediterranean. Here we describe the composition of
a fish assemblage of the rocky slopes at Tremiti Islands, southern Adr
iatic Sea, the vertical distribution at depths from 0 to 42 m, habitat
choice and food use of the different species, and we identify the res
ources along which species are ecologically segregated. Species compos
ition and fish density showed high similarities with the few other ass
emblages that have been censused on the rocky coasts of the Mediterran
ean. We observed a wide overdispersion among species in their use of f
ood and habitat, while very little segregation occurred at the level o
f depth distribution. No coincident use of food, habitat or active per
iods occurred among fish species, and no trophic guilds exist in the t
hree-dimensional niche.