PCR methods for the detection of genetically modified organisms (GMOs) were
developed that can be used for screening purposes and for specific detecti
on of glyphosate-tolerant soybean and insect-resistant maize in food. Proto
cols for amplification of parts of the 35S promoter derived from Cauliflowe
r Mosaic Virus, the NOS terminator derived from Agrobacterium tumefaciens a
nd the antibiotic marker gene NPTII (neomycin-phosphotransferase II) allow
for general screening of foods. PCR/hybridisation protocols were establishe
d for the detection of glyphosate-tolerant RoundUp Ready soybean and insect
-resistant Bt-maize. Besides hybridisation, confirmation of results using r
estriction analysis was also possible. Highly sensitive detection of GMOs -
in most cases 0.01% - could be achieved.