Use of reverse transcription polymerase chain reaction with colorimetric plate hybridization to detect a cytomegalovirus late spliced mRNA in polymorphonuclear leukocytes from renal transplant patients

Authors
Andre, E Imbert-Marcille, BM Cantarovich, D Besse, B Ferre-Aubineau, V Billaudel, S
Citation
E. Andre et al., Use of reverse transcription polymerase chain reaction with colorimetric plate hybridization to detect a cytomegalovirus late spliced mRNA in polymorphonuclear leukocytes from renal transplant patients, DIAG MICR I, 34(4), 1999, pp. 287-291
Citations number
20
Categorie Soggetti
Clinical Immunolgy & Infectious Disease",Microbiology
Journal title
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
ISSN journal
07328893 → ACNP
Volume
34
Issue
4
Year of publication
1999
Pages
287 - 291
Database
ISI
SICI code
0732-8893(199908)34:4<287:UORTPC>2.0.ZU;2-2
Abstract
Human cytomegalovirus replication was evaluated in polymorphonuclear leukoc ytes from ten renal transplant recipients. Three new reverse transcription polymerase chain reactions with plate hybridization suitable for automation were developed for the detection of immediate-early spliced U(L)123 mRNA, early-late pp65 mRNA, and late spliced U(L)22 mRNA. The presence of U(L)22m RNA was found to be significantly associated with the occurrence of cytomeg alovirus (CMV) disease. (C) 1999 Elsevier Science Inc.