SIGNIFICANT INHIBITION OF SPONTANEOUS IGA SECRETION BY SELECTIVE PERIPHERAL-TYPE BENZODIAZEPINE RECEPTOR LIGANDS

The in vitro effect of benzodiazepine (BZ) receptor ligands on the sec retion of immunoglobulin isotypes IgM, IgG, and IgA by human periphera l blood mononuclear cells (PBMCs) was examined. It was found that the specific peripheral-type BZ receptor (PER) ligands (Ro5-4864 and PK 11 195) inhibit the spontaneous secretion of IgA by human PBMCs in a dose -dependent manner, in the micromolar range. The decreased secretion of IgG and IgM induced by these ligands did not reach significant levels . The mixed BZ ligands (diazepam and flunitrazepam) had no consistent or significant effect on the production of the three immunoglobulin is otypes tested in the current study. The central-type ligand (clonazepa m) did not affect IgM, Igc, or IgA secretion. The significant inhibito ry effect of PER ligands was confined to the spontaneous secretion of IgA by human PBMCs, and no such effect was detected in cells stimulate d by pokeweed mitogen to produce immunoglobulins. It seems that PER li gands are capable of suppressing spontaneous IgA secretion, but fail t o affect the augmented production induced by mitogen.