NUCLEAR FACTOR-KAPPA-B ACTIVITY AND ARTERIAL RESPONSE TO BALLOON INJURY

We studied the effect of arterial balloon injury on nuclear factor-kap pa B (NF-kappa B) mobilization and ICAM-1 expression in untreated rats and rats treated with aspirin. Baseline NF-kappa B nuclear binding in smooth muscle cells (SMC) increased two-fold within 6 h after balloon injury. The binding returned to baseline 3 days after injury. Consist ently nuclear staining of p65 active subunit increased in the medial S MC following balloon injury. There was no baseline ICAM-1 expression. Within 3 days after balloon injury there was marked medial ICAM-1 expr ession, that localized to neointima 7 days after injury and to regrowi ng endothelial cells 14 days after injury. Treatment with aspirin inhi bited NF-kappa B nuclear translocation and binding and was associated with reduction of ICAM-1 expression, SMC proliferation and neointimal thickening following balloon injury. These data suggest that transient mobilization of NF-kappa B in vascular SMC after balloon injury media tes ICAM-1 expression and is involved in arterial response to balloon injury. (C) 1997 Elsevier Science Ireland Ltd.