High prevalence of 2-mono- and 2,6-di-substituted Manol-terminating sequences among O-glycans released from brain glycopeptides by reductive alkalinehydrolysis

Citation
Wg. Chai et al., High prevalence of 2-mono- and 2,6-di-substituted Manol-terminating sequences among O-glycans released from brain glycopeptides by reductive alkalinehydrolysis, EUR J BIOCH, 263(3), 1999, pp. 879-888
Citations number
41
Categorie Soggetti
Biochemistry & Biophysics
Journal title
EUROPEAN JOURNAL OF BIOCHEMISTRY
ISSN journal
00142956 → ACNP
Volume
263
Issue
3
Year of publication
1999
Pages
879 - 888
Database
ISI
SICI code
0014-2956(199908)263:3<879:HPO2A2>2.0.ZU;2-9
Abstract
Di- to heptasaccharides isolated from total nondialyzable brain glycopeptid es after release by alkaline borohydride treatment have been subjected to m ass spectrometric and nuclear magnetic resonance spectroscopic analyses sup plemented by TLC-MS analyses of derived neoglycolipids. A family of Manol-t erminating oligosaccharides has been revealed which includes novel sequence s with a 2,6-disubstituted Manol: [GRAPHICS] In contrast to the Manol-terminating HNK-1 antigen-positive chains describe d previously that occur as a minor population [Yuen, C.-T, Chai, W., Lovele ss, R.W., Lawson, A.M., Margolis, R.U. & Feizi, T. (1997) J. Biol. Chern. 2 72, 8924-8931], the above oligosaccharides are abundant. The ratio of these compounds to the classical N-acetylgalactosaminitol-terminating oligosacch arides is about 1:3. Thus, there appears to be in higher eukaryotes a major alternative pathway related to the yeast-type protein O-mannosylation, the enzymatic basis and functional importance of which now require investigati on.