De. Carey et al., SERUM CONTENT OF THE C-PROPEPTIDE OF THE CARTILAGE MOLECULE TYPE-II COLLAGEN IN CHILDREN, Clinical and experimental rheumatology, 15(3), 1997, pp. 325-328
Objective. The C-propeptide of cartilage type II procollagen, together
with the N-propeptide, are removed from newly synthesized procollagen
during collagen fibril assembly in cartilage matrix. The presence and
content of the C-propeptide reflect the synthesis of this molecule. R
ecently, we showed that serum levels of the C-propeptide are increased
in adults with rheumatoid arthritis, pointing to increased synthesis
of this molecule. In this study we examined its content in the sera of
children to determine whether it changes during development. Methods.
Sera were obtained from 44 premature infants (cord blood), 75 childre
n (0-18 years), 14 young adults (18-22 years) and 47 adults (35-60 yea
ts). The concentration of serum C-propeptide of type TI procollagen wa
s determined by a solution phase competitive inhibition radioimmunoass
ay which uses a polyclonal antiserum specific for the bovine and human
C-propeptide. Results. Compared with adults, concentrations of the C-
propeptide of type II procollagen were significantly elevated in child
ren of ages 0-14 years. Concentrations were constant until 10 years of
age (premature infants: 14.5 +/- 1.4 ng/ml, mean +/- SE; 0-10 years:
13.6 +/- 1 ng/ml), In children of ages 10-14 years, during which the p
ubertal growth spurt is ordinarily observed, the mean concentration in
creased (10-14 years: 21.6 +/- 0.7 ng/ml) although not significantly d
ue to the variation between individuals. Concentrations at all ages yo
unger than 14 were significantly greater than those in older adolescen
ts ages 14-18 (6.3 +/- 0.7 ng/ml), young adults (8.4 +/- 2.0 ng/ml) an
d adults (5.7 +/- 0.4 ng/ml). Serum concentrations did not show signif
icant differences with respect to sex, but varied from child to child
at any given age. Conclusions. The measurement of this circulating C-p
ropeptide may be of use in studying the biochemical and physiological
bases of changes in cartilage turnover in children, and abnormalities
thereof.