Microdetermination of proteins by enhanced Rayleigh light scattering spectroscopy with morin

Under acidic conditions, the reaction between 3,5,7,2',4'- pentahydroxyflav one (morin) and proteins enhances the weak light scattering of morin drasti cally. The enhanced light scattering intensity is proportional to the conte nt of proteins. This fact is the basis of a new method for the quantitative analysis of proteins. The linear range is 0.45-7.15 and 0.46-11.14 mu g/mL for BSA and HSA, respectively. The relative standard deviation is 3.76% (n = 12) for BSA in the middle of the linear range. The results of this assay for human serum samples were comparable with those from the traditional me thod (CBB method). There is almost no interfere from amino acids and most o f the metal ions. The scattering spectrum of morin was also discussed.