Cloning and gene structure of rat phosphatidylcholine transfer protein, Pctp

Citation
Mk. Wu et al., Cloning and gene structure of rat phosphatidylcholine transfer protein, Pctp, GENE, 235(1-2), 1999, pp. 111-120
Citations number
37
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENE
ISSN journal
03781119 → ACNP
Volume
235
Issue
1-2
Year of publication
1999
Pages
111 - 120
Database
ISI
SICI code
0378-1119(19990722)235:1-2<111:CAGSOR>2.0.ZU;2-5
Abstract
Phosphatidylcholine transfer protein (PC-TP) Is a cytosolic lipid transfer protein that promotes intermembrane transfer of phosphatidylcholines but no other phospholipids. Although its physiological function remains unknown, phosphatidylcholine transfer protein is enriched in liver and evidence from model systems suggests a role in hepatocellular selection and transport of biliary phospholipids. To facilitate in vivo studies, a cDNA encoding rat PC-TP was cloned by library screening and 5'-rapid amplification of cDNA en ds. Genomic cloning demonstrated the rat Pctp gene spans 10.8 kb and is com prised of six exons. The putative transcription initiation site was identif ied 50 bp upstream of the translation initiation site. Nucleotide sequence analysis of the 5'-flanking region revealed a CAAT- but no TATA-box. Transi ent transfection of a series of 5'-deleted Pctp-promoter-firefly luciferase constructs into Reuber H35 rat hepatoma cells, which express Pctp mRNA, an d Gunn rat fibroblasts, which do not, suggest that cis-acting elements in a 637 bp promoter region contribute to enhanced expression of PC-TP in liver . (C) 1999 Elsevier Science B.V. All rights reserved.