Sublocalization of putative tumor suppressor gene loci on chromosome arm 14q in neuroblastoma

RFLP and microsatellite analysis with 23 polymorphic markers spanning the e ntire long arm of chromosome 14 in 108 neuroblastomas showed allelic loss i n 19 out of 107 (18%) informative tumors, placing 14q among the most freque ntly affected chromosomal regions in neuroblastoma. One minimal deletion re gion could be sublocalized in 17 of 19 cases between markers D14S1 and D14S 16, and a second one between markers D14S17 and D14S23 in band 14q32. Furth ermore, breakpoints in bands 14q23 and 14q12 were detected. These results s uggest the presence of at least two putative tumor suppressor gene loci on chromosome 14. Survival analyses revealed no prognostic impact of allelic l oss of 14q in neuroblastoma. (C) 1999 Wiley-Liss, Inc.