ISOLATION AND CHARACTERIZATION OF A NOVEL GENE FROM HUMAN GLIOBLASTOMA-MULTIFORME TUMOR-TISSUE

Using the technique of DD-PCR (differential display-polymerase chain r eaction) we isolated a novel gene (D2-2) that is overexpressed in glio blastoma multiforme tissue (GMT) as compared to normal brain tissue (N ET), D2-2 is also highly expressed in recurrent glioma, colon tumor me tastatic to brain, breast tumors, prostate tumors and a prostate tumor cell line (LNCaP), Northern blot analysis showed that D2-2 is highly expressed in several tumor cell lines (MOLT lymphoblastic leukemia, SW 480 colorectal adrenocarcinoma, A549 lung carcinoma, HL-60 promyelocyt ic leukemia, S3 HeLa cells, K-562 chronic myelogeneous leukemia and G3 61 melanoma) as compared to NET. Additionally, D2-2 is very highly exp ressed in cell lines derived from glioblastomas, grade IV astrocytomas , normal human fetal astrocytes (NHFA) and glioma. D2-2 is moderately expressed in neuroblastoma, neuroectodermal and medulloblastoma tumor cell lines, D2-2 expression is localized to the frontal robe, occipita l lobe and the cerebellum in the normal brain, Normal tissues such as thyroid, stomach, adrenal cortex, small intestine and pancreas show hi gh expression of D2-2. We also show that D2-2 is expressed 28-fold hig her in fetal brain (20 weeks) than in adult brain, Sequence analysis o f a 2.0-kb fragment for D2-2 shows no homology to known sequences in t he data base. (C) 1997 Wiley-Liss, Inc.