ELECTROCHEMILUMINESCENCE FLOW-INJECTION IMMUNOASSAY FOR ATRAZINE

Antibodies to atrazine were labelled with glucose oxidase and used in colorimetric enzyme linked immunosorbent assays. Transparent aminosila nized indium tin oxide coated glass electrodes were derivatized with a minodextran covalently modified with atrazine caproic acid. The labell ed antibodies were used to investigate the derivatized electrodes colo rimetrically and the electrodes were use in an electrochemiluminescenc e flow injection analyser. Electrochemiluminescence immunoassay for at razine in the range 0-10 ppb showed that it was possible to detect les s than 0.1 ppb, the precautionary limit for pesticides in drinking wat er recommended by the European Commission. (C) 1997 Elsevier Science L imited.